#TUCSEN CAMERA REVIEW SOFTWARE#

The illumination is with LED, so I don't quite know exactly how Koehler is implemented there. I just found the Amscope you are interested in and it already is equipped with plan objectives and is one of their high end scopes. Normally, that may not be an issue, but when photographing hundreds of slides (as from a collection) it, if it can be made to work, will take a long time. I have been toying with the idea of taking several images from a slide and stitching them together to cover a larger area but that's a lot of work to do manually. There, more experience and accessories are required. Then uniformity becomes critical or resourt to dark field is needed otherwise detail is washed out.

#TUCSEN CAMERA REVIEW SOFTWARE#

There are other tricks which can even out the illumination in photoshop or other software except when there is poorly stained tissue. I guess the purpose of my request of the microscope suppliers to see a picture of the illuminated field albeit non uniform was to get a feel for the uniformity. By taking the substage condenser out and using a diffuser there images can be quite uniformly lit (strictly not Koehler)! When Koehler is set correctly as Gekko explained it is normally with a 10x objective. Unfortunately I don't have access to one this microscope in life after the Lab. There seems to be a trade off between the size of the field observed (trinocular with reduction lens) which is seen by the sensor (when I worked at CSIRO I used a 0.3x reduction lens and 10x objective with an old Olypus Vanox which provided an adequate field size together with the sensor area). Thanks Gekko for the excellent explanation of the Koehler process. Hi Gekko, Lorez, JimT, our French colleague, and all the others who have provided comment. In any case, no matter what microscope you ultimately get, I think you'll want your 4x objective to have a flat field, as a minimum. Now I have no idea if those images were actually taken with the microscope in question or not (to my eyes, when I last looked, images supposedly taken with different models appear the same). I am assuming that one is using a standard condenser (NA of 0.9 to 1.4), not a special long focal length condenser for low power use (which may provide Koehler illumination for very low power objectives, but not having used one, I don't know).ДЄstyanax, for what it is worth, if I remember correctly, Amscope's website, at least for some models, displays photomicrographs when you click on the image of the microscope to get a bigger image (or something like that). If, instead of a flip-down condenser you use a diffuser to illuminate the field of view of the 4x objective, then that also means you no longer have Koehler illumination. On the other hand, if the condenser flip down lens is not flipped down, then only a small central part of the field of view is illuminated. By normal conditions I mean that when the flip down condenser lens is flipped down in order to fully illuminate the field of view, the image of the bulb filament is no longer located at the back focal plane of the objective as required for Koehler illumination, and the condenser diaphragm does not control the aperture (it is opened all the way, and the field diaphragm now acts as an aperture diaphragm).

Koehler illumination is not usually provided for very low power objectives (4x, 2x).lorezI mean that the conditions for Koehler illumination no longer obtain with a 4x objective under normal conditions.

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